CGSC Strain#: 7280
Strain Designation: RW82      Source of Strain: R. Woodgate
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ^-, Δ(umuD-umuC)595(::cat), hisG4(Oc), rpsL31(strR), xylA5, mtl-1, ilv-325, thiE1, uvrA6
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = chloramphenicol resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• cat = chloramphenicol acetyl transferase
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Woodgate, R. 1992. Construction of a umuDC operon substitution mutation in Escherichia coli. Mutat.Res. 281:221-225