CGSC Strain#: 7283
Strain Designation: DE2345      Source of Strain: R. Woodgate
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnV44(AS), galK2(Oc), λ^-, umuD1, Rac-0, hisG4(Oc), rpsL31(strR), xylA5, mtl-1, ilv-325, thiE1, uvrA6
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Koch, W.H., D.G. Ennis, A.S. Levine, R. Woodgate 1992. Escherichia coli umuDC mutants: DNA sequence alterations and UmuD cleavage. Mol.Gen.Genet. 233:443-448