CGSC Strain#: 7517
Strain Designation: CAG8160     
Source of Strain: C.A. Gross
Other Designations: CSH123
Sex: Hfr
Point of Origin#: 68
Map Position: 68.60
Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ-,
relA1,
spoT1,
thiC39::Tn10Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- thiC39::Tn10-- From Kleckner strain NK5139. This thiinsertion shown more specifically by PCR to be within thiC, at nucleotide 4,192,143 (minute 90.363 -- Nichols et al. 1998.
- = tetracycline resistant
References: - Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
- Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.