CGSC Strain#: 6821
Strain Designation: CES201     
Source of Strain: C. Shurvinton
Other Designations: DB1256
Sex: F-
Chromosomal Markers: Δ(gpt-proA)62,
lacY1,
tsx-33,
galK2(Oc)
,
λ-,
Rac-0,
sbcB15,
hisG4(Oc)
,
rfbC1,
Δ(recA-srl)306,
srlR301::Tn10,
recB21,
recC22,
rpsL31(strR)
,
kdgK51,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1,
hsdR0Strain Comments: - Related strains are described in the Wyman et al. paper cited below.
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- Δ(recA-srl)306-- removes all, or nearly all, of the recA gene and extends into srlR, but does not remove the Tn10 insertion in srlR
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- = tetracycline resistant
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Wyman, A.R., L.B. Wolfe, D. Botstein 1985. Propagation of some human DNA sequences in bacteriophage Lambda vectors requires mutant Escherichia coli hosts. Proc.Natl.Acad.Sci.USA 82:2880-2884