CGSC Strain#: 7091
Strain Designation: JA221     
Source of Strain: J. Carbon
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
trp-5,
rfbC1,
mgl-51,
recA1,
xylA5,
mtl-1,
thiE1,
hsdR4,
ValRStrain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Chinault, A.C., J. Carbon 1979. Overlap hybridization screening: isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome III. Gene 5:111-126