CGSC Strain#: 5061
Strain Designation: FEJ-1      Source of Strain: W. Epstein
Sex: Hfr    Point of Origin#: 13   Map Position: 84.98   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: leuB6(Am), lacZ4, glnX44(AS), srl-1, rpsL8, mtlA9, thiE1
Strain Comments:

• 1.The srl marker is quite stable. Selection for Srl⁺ works better when the strains are mtl^- because sorbitol has a bit of mannitol in it. Even with
• 2.double mutants, one obtains a bit of background growth; repurification after transduction is essential to obtain reproducible results.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References: