CGSC Strain#: 5207
Strain Designation: ND22      Source of Strain: E. Ohtsubo
Sex: F' Episome/Plasmid: F152-1
   Plasmid Markers/Mutations: gal , aroG , gpmA
   Plasmid Comment: : This derivative of F152 extends from {\it aroG-moaA}, with the region between {\it aroG} and the PO2B F insertion deleted.
Chromosomal Markers: Δ(galK-moaA)34, λ^-, his-87, relA1, rpsL181(strR), spoT1
Strain Comments:

• F152-1 contains the region aroG-moaA and has the region between the F-insertion 2B and aroG deleted.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• strR = streptomycin resist.

References:

• Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.