CGSC Strain#: 5479
Strain Designation: Lin 209     
Source of Strain: E.C.C. Lin
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ΔphoA8,
fadL701(T2R)
,
relA1,
glpR201,
glpD10,
pitA10,
spoT1,
glpF17,
glpK16,
rrnB-2,
mcrB1,
creC510Strain Comments: - phoA8 is a deletion.
- glpD10-- : Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
- glpD10-- : Loss also results in stasis with gluconeogenic C sources, e.g., succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
References: - Richey, DP, EC Lin 1972. Importance of facilitated diffusion for effective utilization of glycerol by Escherichia coli. 112(2):784-90.