CGSC Strain#: 5044
Strain Designation: JM70     
Source of Strain: M.C. Jones-Mortimer
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA2,
lacY1,
glnV44(AS)
,
gal-6,
λ-,
trp-1,
hisG1(Fs)
,
rpsL99(strR)
,
malT1(λ
R)
,
xyl-7,
mtlA2,
cysE52,
ΔargH1,
thiE1Strain Comments: - This strain is sensitive to cysteine: use cystine instead.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnV44(AS) was formerly called supE44
- glnV44(AS) was formerly called su+II
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Fs = Frameshift mutation
- LamR = Lambda-resistant
- strR = streptomycin resist.
References: - Jones-Mortimer, MC 1968. Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping oc cysteineless mutants of E. coli K12. Biochem. J. 110(3):589-95.