CGSC Strain#: 261
Strain Designation: P4X      Source of Strain: Jacob & Wollman
Other Designations: J2
Other Designations: AB261
Other Designations: Hfr Type 2
Sex: Hfr    Point of Origin#: 3   Map Position: 6.80   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: relA1, spoT1, metB1, creC510
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive

References:

• Jacob, F., E. Wollman 1957. Analyse de groupes de liaison genetique de differentes souches donatrices d'Escherichia coli. Compt.Rend.Acad.Sci.Paris 245:1840-1843
• Jacob, F., E. Wollman 1961. Sexuality and genetics of bact., Acad.Press, NY.
• Jacob, F., S. Brenner, F. Cuzin 1963. On the regulation of DNA replication in bacteria. Cold Spr.Harb.Symp.Quant.Biol. 28:329-348