CGSC Strain#: 7570
Strain Designation: VPE42     
Source of Strain: G.J. Barcak
Other Designations: SM10 Lambda-pir pcnB
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
pcnB80,
zad-2084::Tn10,
fhuA21,
cyn-101,
lacY1,
glnX44(AS)
,
LAMpir,
rfbC1,
recA2111::RP4-2Tc::Mu,
glpR200(glpc),
thiE1Strain Comments: - This strain is a pcnB derivative of SM10 Lambda-pir
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- LAMpir-- Allows the replication of plasmids with an R6Kγ origin of replication
- recA2111::RP4-2Tc::Mu-- The RP4-2-Tc::Mu construct contains the transfer genes of the broad host range plasmid RP4
- cyn-101 was formerly called cyn-1
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- = tetracycline resistant
- = kanamycin resistant
- = ampicillin resistance
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- glpc = constitutive expr.glp regulon
References: - Pierson, V.L., G.J. Barcak 1999. Development of E. coli host strains tolerating unstable DNA sequences on ColE1 vectors. Focus 21:18-19