CGSC Strain#: 5550
Strain Designation: PA340      Source of Strain: F. Jacob
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA2, lacY1, glnV44(AS), gal-6, λ^-, gdhA1, hisG1(Fs), rfbC1, galP63, Δ(gltB-gltF)500, malT1(λ^R), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• Δ(gltB-gltF)500-- :Glt^- phenotype in gltB mutant requires presence of a gdhA mutation as well. 0.2% aspartate is a better N-source than glutamate because of uptake(Pahel et al).
• Δ(gltB-gltF)500 was formerly called gltB31
• Δ(gltB-gltF)500 was formerly called aspB21
• Δ(gltB-gltF)500 was formerly called aspB24
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Fs = Frameshift mutation
• L? = assignment uncertain
• LamR = Lambda-resistant

References:

• Reiner, A.M. 1969. Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli. J.Bacteriol. 97:1431-1436
• Berberich, M.A. 1972. A glutamate-dependent phenotype in E. coli K-12: the result of two mutations. Biochem.Biophys.Res.Commun. 47:1498-1503
• Pahel, G, AD Zelenetz, BM Tyler 1978. gltB gene and regulation of nitrogen metabolism by glutamine synthetase in Escherichia coli. J. Bacteriol. 133(1):139-48.
• Castano, I., F. Bastarrachea, A. Covarrubias 1988. gltBDF operon of Escherichia coli. J.Bacteriol. 170:821-827