DG76      Source of Strain:
F- Chromosomal Markers: leuB6
, deoC3Strain Comments:
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- thyA47-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- Am = amber(UAG) mutation
- strR = streptomycin resist.
- Wolf, B, A Newman, DA Glaser 1968. On the origin and direction of replication of the Escherichia coli K12 chromosome. J. Mol. Biol. 32(3):611-29.