CGSC Strain#: 5560
Strain Designation: Lin 188     
Source of Strain: E.C.C. Lin
Sex: F-
Chromosomal Markers: fhuA2 or fhuA22,
glnX44(AS)
,
gal-6,
λ-,
hisG1,
rpsL8 or rplL9(L?)
or rpsL17,
malT1(λ
R)
,
glpD10,
mtlA2,
thiE1,
T2RStrain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- glpD10-- : Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
- glpD10-- : Loss also results in stasis with gluconeogenic C sources, e.g., succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose
- T2R-- : selected for resistance to phage T2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- = strep-resistant
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: - Cozzarelli, NR, WB Freedberg, EC Lin 1968. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J. Mol. Biol. 31(3):371-87.