CGSC Strain#: 5400
Strain Designation: AB301-105      Source of Strain: P. Kindler
Sex: Hfr    Point of Origin#: 21   Map Position: 97.00   Direction of Marker Transfer: Clockwise
Chromosomal Markers: rna-19, suc-11, bio-3, gdhA2, his-95, rnc-105, relA1, spoT1, metB1
Strain Comments:

• 1.References to strain A19 and its derivatives: Reiner 1969 J.Bacteriol. 97:1431, 1437, and 1522; Weatherford et al. 1972 J.Biol.Chem. 247:5404; Studier 1975 J.Bacteriol. 124:307;
• 2.Apirion and Watson 1975 J.Bacteriol. 124:317
• See references for other possible defects in this strain.
• rna-19-- : Also References \\ Weatherford et al. 1972 J.Biol.Chem.247:540 \\ Studier 1975 J.Bacteriol.124:307 \\ Apirion and Watson 1975 J.Bacteriol.124:317
• rna-19-- : Additional references to strain A19 and its derivatives: \\ Gesteland 1966 J.Mol.Biol.16:67 \\ Reiner 1969 J.Bacteriol. 97:1431,1437,1522
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rna-19 was formerly called rns-19

References:

• Reiner, AM 1969. Genetic locus for ribonuclease I in Escherichia coli. J. Bacteriol. 97(3):1522-3.
• Kindler, P., T.U. Keil, P.H. Hofschneider 1973. Isolation and characterization of a ribonuclease 3 deficient mutant of Escherichia coli. Mol.Gen.Genet. 126:53-59
• Studier, FW 1975. Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli. J. Bacteriol. 124(1):307-16.
• Kennell, D. 2002. Processing endoribonucleases and mRNA degradation in bacteria. J.Bacteriol. 184:4645-4657