CGSC Strain#: 7595
Strain Designation: CM1350     
Source of Strain: A. Timms
Sex: F-
Chromosomal Markers: λ-,
tas-1(del-ins)
::kan,
IN(rrnD-rrnE)1,
rph-1Strain Comments: - tas-1(del-ins)::kan-- : 822 basepair segment excised from cloned gene and replaced with a kanamycin-resistant determinant.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = kanamycin resistant
- del-ins = deletion-insertion
References: - Timms, A., B.A. Bridges 1998. Reversion of the tyrosine ochre strain Escherichia coli WU3610 under starvation conditions depends on a new gene tas. Genetics 148:1627-1635