CGSC Strain#: 7600
Strain Designation: ZK126      Source of Strain: R. Kolter
Sex: F- Chromosomal Markers: Δ(argF-lac)169, λ^-, IN(rrnD-rrnE)1, rph-1, tnaA5
Strain Comments:

• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• tnaA5 was formerly called tna-2

References:

• Connell, N., Z. Han, F. Moreno, R. Kolter 1987. An E. coli promoter induced by the cessation of growth. Mol.Microbiol. 1:195-201
• Bohannon, D.E., N. Connell, J. Keener, A. Tormo, M. Espinosa-Urgel, M.M. Zambrano, R. Kolter 1991. Stationary-phase-inducible "gearbox" promoters: differential effects of katF mutations and role of sigma 70. J.Bacteriol. 173:4482-4492