K450      Source of Strain:
F- Chromosomal Markers: nusB5
, rph-1Strain Comments:
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- strR = streptomycin resist.
- Friedman, D., M. Baumann, L.S. Baron 1976. Cooperative effects of bacterial mutations affecting lambda N gene expression. I. Isolation and characterization of a nusB mutant. Virology 73:119-127