CGSC Strain#: 5516
Strain Designation: Lin 242     
Source of Strain: E.C.C. Lin
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ΔphoA8,
fadL701(T2R)
,
relA1,
glpR2(glpc),
pitA10,
spoT1,
glpK23(p)
,
rrnB-2,
mcrB1,
creC510Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- glpc = constitutive expr.glp regulon
- p = promoter, regulatory region
- T2R = T2 Resistant
References: - Berman-Kurtz, M., E.C.C. Lin, D.P. Richey 1971. Promoter-like mutant with increased expression of the glycerol kinase operon of Escherichia coli. J.Bacteriol. 106:724-731
- Richey, DP, EC Lin 1972. Importance of facilitated diffusion for effective utilization of glycerol by Escherichia coli. 112(2):784-90.