CGSC Strain#: 5691
Strain Designation: NI748      Source of Strain: M. Gellert
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, lacY1, glnV44(AS), λ^-, thyA6, malA49, gyrB41(Const), thiE1, deoC1, ilv-655, λ^R
Strain Comments:

• The locus of the Cou^R mutation described in the paper below is now designated gyrB.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• λ^R-- Lambda resistant, locus unknown.
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Const = constitutive

References:

• Gellert, M., M.H. O'Dea, A. Itoh, J.-I. Tomizawa 1976. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc.Natl.Acad.Sci.USA 73:4474-4478