CGSC Strain#: 5523
Strain Designation: DF220     
Source of Strain: D. Fraenkel
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ompF627(T2R)
,
gapA1(Unst)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - This strain grows very poorly on glycerol plus malate unless the medium is supplemented with casamino acids. The gap-1 marker reverts at a relatively high rate.
- gapA1(Unst)-- reverts at a relatively high rate.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
- Unst = unstable
References: - Hillman, JD, DG Fraenkel 1975. Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. J. Bacteriol. 122(3):1175-9.