CGSC Strain#: 5807
Strain Designation: M7025      Source of Strain: J.R. Beckwith
Sex: F- Chromosomal Markers: lacZ90(Oc), λ^-, e14-, relA1, rpsL150(strR), spoT1
Strain Comments:

• lacZ90(Oc)-- is the allele from Paris strain Hfr3000 X90. It is an ochre mutation, in `interval 16' of Newton et al. JMB 14:290(1965).
• e14--- : Loss of the e14 element makes the strain McrA^-.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296