CGSC Strain#: 5828
Strain Designation: MF634      Source of Strain: M.G. Sunshine
Sex: F- Chromosomal Markers: thr-1, dnaJ259(ts), leuB6(Am), fhuA21, lacY1, glnV44(AS), λ^-, e14-, rfbC1, glpR200(glp^c), thiE1
Strain Comments:

• dnaJ259(ts)-- This strain is Ts only at 44.5 degrees C, at which temperature the parent strain (C600) is barely viable. dnaJ259 has been called groPC259, groPF259, and grpF259.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• e14--- : Loss of the e14 element makes the strain McrA^-.
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• glp^c = constitutive expr.glp regulon
• ts = Temperature Sensitive

References:

• Sunshine, M., M. Feiss, J. Stuart, J. Yochem 1977. A new host gene (groPC) necessary for lambda DNA replication. Mol.Gen.Genet. 151:27-34
• Yochem, J, H Uchida, M Sunshine, H Saito, CP Georgopoulos, M Feiss 1978. Genetic analysis of two genes, dnaJ and dnaK, necessary for Escherichia coli and bacteriophage lambda DNA replication. Mol. Gen. Genet. 164(1):9-14.