CGSC Strain#: 7692
Strain Designation: W3110tnaA2trpEFBR19      Source of Strain: C. Yanofsky
Sex: F- Chromosomal Markers: λ^-, trpE719(fbR), IN(rrnD-rrnE)1, rph-1, tnaA5
Strain Comments:

• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• tnaA5 was formerly called tna-2
• trpE719(fbR) was formerly called trpEFBR19
• trpE719(fbR) was formerly called trpE19^FBR
• fbR = feedback resistant

References:

• Doolittle, W.F., C. Yanofsky 1968. Mutants of Escherichia coli with an altered tryptophanyl-transfer ribonucleic acid synthetase. J.Bacteriol. 95:1283-1294
• Yanofsky, C., R.L. Kelley, V. Horn 1984. Repression is relieved before attenuation in the trp operon of Escherichia coli as tryptophan starvation becomes increasingly severe. J.Bacteriol. 158:1018-1024