CGSC Strain#: 6025
Strain Designation: KS5     
Source of Strain: K. Shimada
Sex: Hfr
Point of Origin#: 1
Map Position: 96.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: Δ(galK-uvrB)45,
λ-,
e14-,
relA1,
spoT1,
thiE1Strain Comments: - The use of the symbol sup in the paper cited above is confusing. This strain does not carry a suppressor.
- e14--- : Loss of the e14 element makes the strain McrA-.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- thiE1 was formerly called thi-1
References: - Shimada, K, RA Weisberg, ME Gottesman 1973. Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K12. J. Mol. Biol. 80(2):297-314.
- Fimmel, AL, RE Loughlin 1977. Isolation of a lambdadcys transducing bacteriophage and its use in determining the regulation of cysteine messenger ribonucleic acid synthesis in Escherichia coli K-12. J. Bacteriol. 132(3):757-63.