CGSC Strain#: 6057
Strain Designation: JF496     
Source of Strain: J. Felton
Sex: Hfr
Point of Origin#: 19
Map Position: 84.30
Direction of Marker Transfer: Clockwise
Chromosomal Markers: ubiF411,
asnB50::Tn5,
nagB2,
relA1,
spoT1,
rbs-4,
asnA31,
metB1,
bglR13Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- asnA31-- Reported to be an amber mutation
- = kanamycin resistant
References: - Felton, J, S Michaelis, A Wright 1980. Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli. J. Bacteriol. 142(1):221-8.