CGSC Strain#: 6058
Strain Designation: JF491      Source of Strain: J. Felton
Sex: F- Chromosomal Markers: glnX44(AS), asnB50::Tn5, nagB2, gltA6, galK30, λ^-, pyrD36, rpsL129(strR), rbs-4, asnA31, thiE1, bglR13
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• asnA31-- Reported to be an amber mutation
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = kanamycin resistant
• AS = amber(UAG) suppressor
• strR = streptomycin resist.

References:

• Felton, J, S Michaelis, A Wright 1980. Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli. J. Bacteriol. 142(1):221-8.