CGSC Strain#: 6084
Strain Designation: HMS83     
Source of Strain: C.C. Richardson
Sex: F-
Chromosomal Markers: polB100,
lacZ53(Am)
,
λ-,
thyA36,
IN(rrnD-rrnE)1,
rpsL151(strR)
,
polA1(Am)
,
rha-5,
deoC2,
lys-34Strain Comments: - lacZ53(Am)-- is the Paris lac-Y14 amber.
- thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- polA1(Am)-- normal levels exonuc.;premature term.of polymerase <1% WT level. MMSS,UVS, viable at all temps. Grindley MGG143
- Am = amber(UAG) mutation
- strR = streptomycin resist.
References: - Campbell, JL, L Soll, CC Richardson 1972. Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II. Proc. Natl. Acad. Sci. U.S.A. 69(8):2090-4.
- Campbell, JL, H Shizuya, CC Richardson 1974. Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12. J. Bacteriol. 119(2):494-9.