CGSC Strain#: 6115
Strain Designation: RH5     
Source of Strain: R.L. Hanson
Sex: F-
Chromosomal Markers: lacY1,
tsx-29,
glnX44(AS)
,
galK2(Oc)
,
λ-,
pnt-2::Tn5,
rpsL700(strR)
,
mtl-1,
argE3(Oc)
Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- = kanamycin resistant
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Hanson, RL, C Rose 1980. Effects of an insertion mutation in a locus affecting pyridine nucleotide transhydrogenase (pnt::Tn5) on the growth of Escherichia coli. J. Bacteriol. 141(1):401-4.