CGSC Strain#: 5871
Strain Designation: CA358      Source of Strain: D. Zipser
Other Designations: ZIP195
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ521(UGA), λ^-, e14-, trpA49(Am), relA1, spoT1
Strain Comments:

• lacZ521(UGA)-- is an UGA mutation in region 13 of the lacZ gene, as described by Zipser et al. 1970.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• trpA49(Am)-- is the amber mutation formerly called trp^-_8am by Brenner.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Am = amber(UAG) mutation
• UGA = opal(UGA) mutation

References:

• Zipser, D. 1967. J.Mol.Biol. 29:441-0
• Zipser, D, S Zabell, J Rothman, T Grodzicker, M Wenk 1970. Fine structure of the gradient of polarity in the z gene of the lac operon of Escherichia coli. J. Mol. Biol. 49(1):251-4.