CGSC Strain#: 7697
Strain Designation: JMB9hsdR-M+Δ(trpF)     
Source of Strain: C. Yanofsky
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
glnX44(AS)
,
galK0,
λ-,
ΔtrpC721(trpF),
xylA5,
mtl-1,
thiE1,
hsdR2,
hsdR-Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- ΔtrpC721(trpF)-- : This mutation deletes the TrpF, PRA isomerase, portion of the trpC gene, leaving an artifically monofunctional IGP synthase gene. (C.Yanofsky Lab)
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- trpF(del) = del.of PRA Isomerase (F) dom.
References: - Sterner, R., A. Dahm, B. Darimont, A. Ivens, W. Liebl, K. Kirschner 1995. (beta-alpha)8-barrel proteins of tryptophan biosynthesis in the hyperthermophile Thermotoga maritima. EMBO J. 14:4395-4402
- Darimont, B., C. Stehlin, H. Szadkowski, K. Kirschner 1998. Mutational analysis of the active site of indoleglycerol phosphate synthase from Escherichia coli. Protein Sci 7:1221-1232