JG110      Source of Strain:
F- Chromosomal Markers: λ-
, deoC2Strain Comments:
- thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- polA5-- polymerase altered pH opt., altered template specificity, lower overall activity; Exo-greater than normal levels. see G. and K.
- Grindley, ND, WS Kelley 1976. Effects of different alleles of the E. coli K12 pol A gene on the replication of non-transferring plasmids. Mol. Gen. Genet. 143(3):311-8.