CGSC Strain#: 6185
Strain Designation: NK6045      Source of Strain: N. Kleckner
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: Δ(gpt-lac)5, λ^-, e14-, purM78::Tn10, relA1, spoT1, thiE1
Strain Comments:

• The deletion comes from strain Hfr 3000 X111 of Jacob.
• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• purM78::Tn10 was formerly called purG78::Tn10
• thiE1 was formerly called thi-1
• = tetracycline resistant

References: