CGSC Strain#: 7856
Strain Designation: BW5328/pAH57     
Source of Strain: B.L. Wanner
Sex: F-
Episome/Plasmid: pAH57
Plasmid Markers/Mutations: LAMcI857(ts)
,
repA101(ts)
,
oriR101,
λpR-xis,
bla(ApR)
,
intλ,
bla ,
cI Chromosomal Markers: Δ(argF-lac)169,
recA1,
rpoS396(Am)
,
rph-1,
hsdR514,
rob-1,
creC510Strain Comments: - Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
- Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Am = amber(UAG) mutation
- Const = constitutive
- ApR = ampicillin resistant
- ts = Temperature Sensitive
References: - Haldimann, A, BL Wanner 2001. Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. J. Bacteriol. 183(21):6384-93.