CGSC Strain#: 6194
Strain Designation: DF1020     
Source of Strain: D. Fraenkel
Sex: F-
Chromosomal Markers: pro-82,
glnX44(AS)
,
λ-,
ΔpfkB201,
recA56,
endA1,
Δ(rhaD-pfkA)200,
thiE1,
hsdR17Strain Comments: - Reference below is to related strains DF1010. Derivation of DF1020 follows the same steps as DF1010, but the starting strain was MM294A rather than K10.
- There are problems verifying the pfk- phenotype of this strain. Suggest using RL257 instead.
- pro-82-- is in proA or proB.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- recA56-- Amino acid substitution Arg 60 Cys
- endA1-- G to A transition mutation resulting in E208K amino acid substitution.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- = good miniprep dsDNA quality
- AS = amber(UAG) suppressor
References: - Daldal, F. 1983. Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme. J.Mol.Biol. 168:285-305