CGSC Strain#: 6361
Strain Designation: NK6069     
Source of Strain: N. Kleckner
Sex: F-
Chromosomal Markers: pro-82,
glnX44(AS)
,
λ-,
endA1,
thiE1,
purD81(KanR)
::Tn5,
hsdR17Strain Comments: - This is a derivative of strain MM294A.
- This strain requires histidine for growth on supplemented minimal medium (and addition of methionine as well enhances growth).
- pro-82-- is in proA or proB.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- endA1-- G to A transition mutation resulting in E208K amino acid substitution.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- = good miniprep dsDNA quality
- = kanamycin resistant
- AS = amber(UAG) suppressor
- KanR = kanamycin resistance
References: