CGSC Strain#: 6372
Strain Designation: SJ2      Source of Strain: S. Jakowski
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: panB6, zad-220::Tn10, λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• The order is aceEF, pan, TetR. This strain appears to be no longer Hfr.
• zad-220::Tn10-- cotransduces 96-98% with panD. The insertion is in orf yadC (at nucleotide 150,100, minute 3.235).
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• zad-220::Tn10 was formerly called yadC
• = tetracycline resistant

References: