CGSC Strain#: 5966
Strain Designation: DF576      Source of Strain: D. Fraenkel
Sex: F- Chromosomal Markers: glnX44(AS), λ^-, relA1, pgk-2, rpsL104, malA, malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• 1.This strain can be grown in a minimal medium containing malate (25mM), glycerol (12.5 mM) and casamino acids (0.1%) It should not be grown in the presence of glucose, which selects for revertants.
• 2.Reference: Hillman and Fraenkel 1975 J.Bacteriol.122:1175
• Donor markers transferred early in the cross, but not near the selected region and not been tested for: fhuA22, garB10, creC570, mcrB1, rrnB-2, spoT1, pit-10. relA1 is a donor marker near the selected argA⁺ and very likely to be
• The markers shown with ? marks are carried by the recipient parent in regions not selected for, but introduced early by the Hfr. λ^- and glnV44 are recipient markers in a region transferred very late, so are likely to be present.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• pgk-2-- : Glucose will select for revertants of pgk-2. (Mutations in the eno, gap, and pgk genes all revert and must be monitored closely.)
• pgk-2-- Does not allow growth on glycerol alone or oxidative substrate alone; cannot grow on sugars; glucose inhibits growth on other substrates. Can grow on glycerol plus oxidative substrate (malate)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• pgk-2 was formerly called pgk-1
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• = strep-resistant
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Thomson, J, PD Gerstenberger, DE Goldberg, E Gociar, A Orozco de Silva, DG Fraenkel 1979. ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt. J. Bacteriol. 137(1):502-6.