CGSC Strain#: 7895
Strain Designation: XA92      Source of Strain: J.H. Miller
Other Designations: XA102 but Met+
Sex: F- Chromosomal Markers: Δ(gpt-lac)5, glnX44(AS), glnV89(AS), λ cI⁺, ara(FG), gyrA-0(NalR), metB-, argE-(Am), rpoB0(rifR), thiE1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnV89(AS)-- : This mutation is shown in the Promega Technical Bulletin on the Interchange System, but not in the parental strains.
• λ cI⁺-- is shown in Promega Technical Bulletin, but not in parental strains.
• argE-(Am)-- is suppressible by all of the characterized amber and ochre suppressors in Miller et a. 1977
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.
• rifR = rifamycin resistance

References:

• Miller, J.H., D. Ganem, P. Lu, A. Schmitz 1977. Genetic studies of the lac repressor. I. Correlation of mutational sites with specific amino acid residues: construction of a colinear gene-protein map. J.Mol.Biol. 109:275-301