HC194      Source of Strain:
Sakakibara & MizukamiSex:
Hfr Point of Origin#:
2A Map Position:
12.20 Direction of Marker Transfer:
Counterclockwise Chromosomal Markers: fhuA22
, metB1Strain Comments:
- The dnaN allele is called dnaN59 in the paper cited.
- thyA148-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- T2R = T2 Resistant
- ts = Temperature Sensitive
- Sakakibara, Y., T. Mizukami 1980. A temperature-sensitive Escherichia coli mutant defective in DNA replication: dnaN, a new gene adjacent to the dnaA gene. Mol.Gen.Genet. 178:541-553