CGSC Strain#: 6845
Strain Designation: HC194     
Source of Strain: Sakakibara & Mizukami
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ompF627(T2R)
,
relA1,
thyA148,
dnaN159(ts)
,
metB1Strain Comments: - The dnaN allele is called dnaN59 in the paper cited.
- thyA148-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- T2R = T2 Resistant
- ts = Temperature Sensitive
References: - Sakakibara, Y., T. Mizukami 1980. A temperature-sensitive Escherichia coli mutant defective in DNA replication: dnaN, a new gene adjacent to the dnaA gene. Mol.Gen.Genet. 178:541-553