CGSC Strain#: 8208
Strain Designation: BW19094/pLD53     
Source of Strain: B.L. Wanner
Sex: F-
Episome/Plasmid: pLD53
Plasmid Markers/Mutations: oriR6Kγ,
MCS,
tetA,
oriTRP4,
bla-1(ApR,dPstI),
tetR Chromosomal Markers: Δ(codB-lacI)3,
ΔphoA532,
ΔuidA3::pir+,
Δ(phnP-phnD)3330( phnC?)Strain Comments: - Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
- Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
- Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
- ΔuidA3::pir+-- Allows the replication of plasmids with an R6Kγ origin of replication
- Δ(codB-lacI)3 was formerly called Δlac74
- Δ(codB-lacI)3 was formerly called Δ(lac)3
- Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
- Δ(codB-lacI)3 was formerly called X74
- Δ(codB-lacI)3 was formerly called DE(lac)6
- ΔuidA3::pir+ was formerly called uidA(Δ MluI)::pir+
- phnC? = may also delete phnC
- = tetracycline resistant
- ApR = ampicillin resistant
- dPstI = PstI site deleted
References: - Metcalf, W.W., W. Jiang, B.L. Wanner 1994. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6Kgamma origin plasmids at different copy numbers. Gene 138:1-7