CGSC Strain#: 8226
Strain Designation: BW19094/pWM7     
Source of Strain: B.L. Wanner
Sex: F-
Episome/Plasmid: pWM7
Plasmid Markers/Mutations: oriR6Kγ,
bla(ApR)
,
MCS,
tetA,
oriTRP4,
tetR Plasmid Comment: Not accessioned. Glycerol cultures only. Same host as pLD53 and not as good, since bla has PstI site (which has been deleted from pLD53).
Chromosomal Markers: Δ(codB-lacI)3,
ΔphoA532,
ΔuidA3::pir+,
Δ(phnP-phnD)3330( phnC?)Strain Comments: - Not assigned a CGSC No. because pWM53, in the same host, is more useful because of its deletion of PstI site in bla. Glycerol cultures only.
- Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
- Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
- Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
- ΔuidA3::pir+-- Allows the replication of plasmids with an R6Kγ origin of replication
- Δ(codB-lacI)3 was formerly called Δlac74
- Δ(codB-lacI)3 was formerly called Δ(lac)3
- Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
- Δ(codB-lacI)3 was formerly called X74
- Δ(codB-lacI)3 was formerly called DE(lac)6
- ΔuidA3::pir+ was formerly called uidA(Δ MluI)::pir+
- phnC? = may also delete phnC
- = tetracycline resistant
- ApR = ampicillin resistant
References: - Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.