CGSC Strain#: 5240
Strain Designation: 30SO      Source of Strain: F. Jacob
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ43(Fs), λ^-, relA1, spoT1, thiE1
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• Fs = Frameshift mutation

References:

• Luria, S.E., J.N. Adams, R.C. Ting 1960. Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology 12:348-390
• Beckwith, J.R., A.B. Pardee, R. Austrian, F. Jacob 1962. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J.Mol.Biol. 5:618-634
• Bachmann, BJ 1972. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol.Rev. 36(4):525-57.