CGSC Strain#: 7781
Strain Designation: K529     
Source of Strain: M. Russel
Sex: F-
Episome/Plasmid: pLM2
Plasmid Markers/Mutations: kan,
tet(Am)
,
ampR(Am)
,
tetA ,
bla ,
KanR Chromosomal Markers: lacZ125(Am)
,
λ-,
e14-,
trpA49(Am)
,
relA1,
spoT1Strain Comments: - lacZ125(Am)-- is the amber mutation from Zipser's strain NG125 and Brenner's CA274. It was formerly called lac125am by Brenner. In `interval 6' of Newton et al. JMB 14:290 (1965).
- e14--- : Loss of the e14 element makes the strain McrA-.
- trpA49(Am)-- is the amber mutation formerly called trp-8am by Brenner.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- Am = amber(UAG) mutation
- = kanamycin resistant
- Am = amber(UAG) mutation
References: - Mindich, L., C.J. Cohen, M. Weisburd 1976. Isolation of nonsense suppressor mutants of Pseudomonas. J.Bacteriol. 126:177-182