CGSC Strain#: 5262
Strain Designation: Hfr 3000 X90     
Source of Strain: F. Jacob
Sex: Hfr
Point of Origin#: 1
Map Position: 96.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ90(Oc)
,
λ-,
e14-,
relA1,
spoT1,
thiE1Strain Comments: - lacZ90(Oc)-- is the allele from Paris strain Hfr3000 X90. It is an ochre mutation, in `interval 16' of Newton et al. JMB 14:290(1965).
- e14--- : Loss of the e14 element makes the strain McrA-.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- thiE1 was formerly called thi-1
- Oc = ochre(UAA) mutation
References: - Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296
- Beckwith, J.R., E.R. Signer 1966. Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi 80. J.Mol.Biol. 19:254-265