CGSC Strain#: 8164
Strain Designation: BW21116/pLD78      Source of Strain: B.L. Wanner
Sex: F- Episome/Plasmid: pLD78
   Plasmid Markers/Mutations: oriR6K_γ, bla(ApR), tetA, oriT_RP4, tetR, rhaSp-rhaD', rhaBp
Chromosomal Markers: Δ(argF-lac)169, ΔuidA3::pir⁺, rpoS396(Am), rph-1, hsdR514, rob-1, creC510
Strain Comments:

• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• ΔuidA3::pir⁺-- Allows the replication of plasmids with an R6Kγ origin of replication
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• ΔuidA3::pir⁺ was formerly called uidA(Δ MluI)::pir⁺
• Am = amber(UAG) mutation
• Const = constitutive
• = tetracycline resistant
• ApR = ampicillin resistant

References:

• Haldimann, A., L.L. Daniels, B.L. Wanner 1998. Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon. J.Bacteriol. 180:1277-1286