CGSC Strain#: 7029
Strain Designation: KY1445      Source of Strain: T. Yura
Sex: F- Chromosomal Markers: [araD139]_B/r, Δ(argF-lac)169, λ^-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), zhh-50::Tn10, rbsR22, Δ(fimB-fimE)632(::IS1), deoC1
Strain Comments:

• zhh-50::Tn10 cotransduces 70% with rpoH.
• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• zhh-50::Tn10-- cotransduces 80% with rpoH. It is in the intergenic region between ugpB and livF, at nucleotide 3,590,125 (77.386 min.).
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• zhh-50::Tn10 was formerly called zhf-5
• = tetracycline resistant
• ::IS1 = insertion of IS1
• fruA25 = former designation
• strR = streptomycin resist.

References:

• Tobe, T., K. Ito, T. Yura 1984. Isolation and physical mapping of temperature-sensitive mutants defective in heat shock induction of proteins in Escherichia coli. Mol.Gen.Genet. 195:10-16