CBK103      Source of Strain:
F- Chromosomal Markers: λ-
, deoC2Strain Comments:
- thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- cysG98::Tn5-- The Tn5 insertion is in the 3' end of the gene, disrupting only the methylase function of the enzyme. The oxidase and ferrochelatase fcts. are unaffected. T.Leustek, see JBC 1997 272:2744-52
- = kanamycin resistant
- Am = amber(UAG) mutation