CGSC Strain#: 4917
Strain Designation: U3     
Source of Strain: A. Garen
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
phoA14(Oc)
,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- Oc = ochre(UAA) mutation
- T2R = T2 Resistant
References: - Gallucci, E., A. Garen 1966. Suppressor genes for nonsense mutations. II. The Su-4 and Su-5 suppressor genes of E. coli. J.Mol.Biol. 15:193-200