CGSC Strain#: 7971
Strain Designation: GM1674     
Source of Strain: M.G. Marinus
Sex: F'
Episome/Plasmid: F128-20
Plasmid Markers/Mutations: lacIp-4000(lacIQ),
ΔlacZ58(M15)
Chromosomal Markers: Δ(gpt-lac)5,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
dcm-6,
dam-3,
thiE1Strain Comments: - Δ(gpt-lac)5-- is the deletion Δ( pro-lac)X111 of Jacob and originated in the strain Hfr 3000 X111.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- dcm-6-- has a Gln to Arg mutation,also a TGG to TGA termination mutation,which results in dcm-vsr message degradation and causes a Vsr- phenotype (Dar 1993).
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- M15 = alpha-complemented allele
References: